Scar

Part:BBa_K2042019:Design

Designed by: Toky RATOVOMANANA   Group: iGEM16_Evry   (2016-09-11)


Spacer in between promoter and coding sequence


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 1
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

This sequence was designed as a spacer to prevent errors in transcription by placing the RBS close to the promoter. Formation of a hairpin structure or self complementary sequences was avoided.

Source

Synthetic synthesis

References